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- Cayman
- 37396-1 mg
- 2025年07月21日
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文献和实验Deproteination using phenol/chloroform
'Phenol' as used is Analar grade. Phenol should be melted at 65℃,8-hydroxyquinoline added to a final concentration of 0.1%, and equilibrated three times with an equal volume of 1M Tris.HCl, pH 7.0. The final Tris wash is replaced with TE (10
Deproteination using phenol/chloroform
to a final concentration of 0.1%, and equilibrated three times with an equal volume of 1M Tris.HCl, pH 7.0. The final Tris wash is replaced with TE (10mM Tris, 1mM EDTA, pH8.0) and the phenol stored in the dark at 4℃. 8-hydroxyquinoline is added
troponin蛋白纯化 Protein purification: troponins
regulatory mechanism for muscle contration. Material Source: skeletal muscle ether powder Equipment: • centrifuge • Cibaron Blue-sephacryl column (5 x 50 cm) • DEAE-sephadex A-50 column (2.5 x 25 cm) • CM-sephadex C-50-120 column
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