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文献和实验Electrotransfection with Intracellular Buffer
. Nevertheless, the pores that are induced by the electroporation technique (3 –5 ) can be visualized by rapid-freezing electron microscopy techniques (6 ) and allow a direct contact between the cytoplasm of the cells and the electroporation medium. The components
Buffers and Reagents: Bicarbonate/carbonate coating buffer (100 mM) - antigen or antibody should be diluted in coating buffer to immobilize them to the wells: 3.03 g Na2CO3, 6.0 g NaHCO3 (1000 ml distilled water) pH 9.6, PBS: 1.16 g Na
holder on the lab bench and pour about 10 ml of buffer or acetic acid into the cuvette. Unfixed gels still in the buffer that you used to wash them will work best for this purpose. If the gels have been fixed, be careful of spills and minimize contact
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