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文献和实验Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
antibiotic (see Step 51). Ligation buffer (10X) Dilute the 10X stock to 1.15X for Step 18. Magnetic beads, Protein A-conjugated Magnetic beads, Protein G-conjugated PCR buffer (10X) PCR primers, T3 and T7 Phenol
Mouse p27 PCR Using Gitschier Buffer
: 1 M Tris pH 8.8 (do not use pH meter, store at room temp.) 1.23 g Tris HCl 5.13 g Tris base q.s. 50 mL with H2 O KG-1 (10x) (Store frozen) Amt [final] 8.3 mL 1M (NH
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