Anti-HLA-DR (α and β chains) E

（共享）ELISA原理与实验方法

thousands of colored products. Thus, in ELISA, antigens are detectable when present in only nanogram and picogram quantities. One form of the ELISA is carried out in a microtiter plate which is used as the solid phase for adsorption of antigen (Figure

使用免疫学方法检测sia α 2-3糖链

1 前言 唾液酸主要存在于高等动物的细胞表面，是糖蛋白、糖脂等复合糖的组成成分，担任重要的生物学功能1）。另外，众所周知唾液酸糖链是流感病毒的受体，它像病原性微生物一样被人体识别。 传统方法检测α 2-3结合型唾液酸糖链的非还原糖链末端通常使用MAM或者MAH凝集素。这里的凝集素末端具有Sia α 2-3 Gal β 1- 4GlcNAc 结构，能与N结合型糖链的3链、4链紧密结合2）。但凝集素对糖脂反应性低，对单糖、二糖的结合性低，且难以广泛的检测与不同苷元

ELISA

on a polystyrene ELISA plate, and antibody is allowed to bind to the protein. The bound antibody is detected by the addition of a second antibody conjugated to an enzyme and an enzyme substrate with color detector is added. The relative intensity of the color