Thioredoxin Fluorometric Activ

Cayman的硫氧还蛋白荧光活性检测试剂盒提供了一种检测细胞裂解物中Trx活性的便捷方法。Trx活性的测量基于Trx对伊红标记的胰岛素二硫化物的还原。胰岛素二硫化物的还原会导致伊红释放，荧光增加，可在560nm处检测到。Trx在此过程中被氧化，氧化的Trx被Trx还原酶回收到还原状态。在Trx活性速率受限的情况下，荧光增加的速率与样品中的Trx活性成正比。Cayman's Thioredoxin Fluorescent Activity Assay Kit provides a convenient method of detecting Trx activity in cell lysate. Measurement of the Trx activity is based on the reduction of eosin-labeled insulin disulfides by Trx. The reduction of insulin disulfides results in a release of eosin and an increase in fluorescence that can be detected at 560 nm. Trx becomes oxidized during this process, and oxidized Trx is recycled back to the reduced state by Trx reductase. Under circumstances in which the Trx activity rate is limiting, the rate of fluorescence increase is directly proportional to the Trx activity in the sample.分子式分子量