HDAC Fluorometric Activity Ass

HDAC催化水解去除组蛋白赖氨酸残基上的乙酰基，导致染色质凝聚和染色体DNA的转录抑制。因此，HDAC抑制可使DNA构象松弛，从而实现转录激活。关键基因的转录抑制将HDAC活性与细胞生长和肿瘤发展联系起来。Cayman的HDAC活性测定法提供了一种快速、基于荧光的方法来测量I类和II类HDAC活性，无需放射性、提取或色谱分析。该过程仅需两个简单的步骤，均在同一微孔板中执行。使用激发波长在340-360nm之间、发射波长在440-465nm之间的酶标仪分析荧光反应产物。HDACs catalyze the hydrolytic removal of acetyl groups from histone lysine residues resulting in chromatin condensation and transcriptional repression of chromosomal DNA. Thus, HDAC inhibition allows the conformation of DNA to be relaxed and transcriptional activation to ensue. Transcriptional repression of key genes has linked HDAC activity to cell growth and tumor development. Cayman's HDAC Activity Assay provides a fast, fluorescence-based method for measuring Class I and II HDAC activity that eliminates radioactivity, extraction, or chromatography. The procedure requires only two easy steps, both performed in the same microplate. The fluorescent reaction product is analyzed using a plate reader with excitation wavelengths between 340-360 nm and emission wavelengths between 440-465 nm.