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文献和实验Dual- and Triple-Color Fluorospot
can also be adjusted to single staining by using reagents for only one cytokine. 1. Dual-Color Fluorospot 1) Preparation of the plates and coating for double staining (sterile conditions) a. Pre-wet a sterile 96-well PVDF plate with 20 μl
Human IL-2 Enzyme Immunoassay (EIA)
-2 EIA is an in vitro enzyme-linked immunosorbent assay for quantitatively detecting human IL-2 in biological fluids (e.g. serum, plasma) and in cell culture supernatants. The kit consists of a 96 well microtiter plate that has been precoated with anti-IL
Methods of Measuring Antibodies in Sera/Plasma, Ascites, and Tissue-Culture Media
) (1 ,2 ). In these procedures, the wells of 96-well plates or strip plates are coated with antigen. Antibody that binds to the antigen is detected by its reaction with an anti-immunoglobulin that has been conjugated to a reporter molecule, usually horseradish peroxidase (HRP
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