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文献和实验Eccles:Protein Lysates from Tissue
Cell Lysis Buffer 5mL 0.1M Tris HCl pH 8 (10mM) 0.44g NaCl (150mM) 0.02g EDTA (1mM) 0.5mL nonidet P40 (1% w/v) 0.05g SDS (0.1% w/v) Make up to 50mL with MQH2 O and filter sterilise. 20x Complete mini Protease Inhibitor
Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
. 21. Incubate for 30 min at room temperature. Enrichment of Ligation Fragments Containing the Protein of Interest in the Complex 22. Remove 10 µL from the sample (from Step 21). Store at -20°C. This sample will serve
Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
Protein microarrays offer a global perspective on the function of expressed gene products. However, technical issues related to the stability and dynamic range of microarrays printed with purified protein have hampered their widespread adoption. Taking
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