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文献和实验Calpain activity can be assayed by a variety of methods which almost invariably rely on measuring the liberation of trichloroacetic acid (TCA)-soluble peptides from a protein substrate, usually casein. The soluble peptides
Fluorometric Quantitation of RNA or DNA
Materials DNA: Höchst 33258 (High range solution): (Invitrogen H1398 or Sigma B1155 ) 1µg/mL in 1x TNE Filter and s tore at 4ºC in an amber bottle. 10x TNE stock: 100 mM Tris, 2 M NaCl, 10 mM EDTA, pH7.4. Store at room
Protein Precipitation Protocols
TCA-DOC For precipitation of very low protein concentration 1) To one volume of protein solution, add 1/100 vol. of 2% DOC (Na deoxycholate, detergent). 2) Vortex and let sit for 30min at 4ºC. 3) Add 1/10 of Trichloroacetic acid (TCA) 100
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