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- 2025年07月21日
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文献和实验Calpain activity can be assayed by a variety of methods which almost invariably rely on measuring the liberation of trichloroacetic acid (TCA)-soluble peptides from a protein substrate, usually casein. The soluble peptides
Fluorometric Quantitation of RNA or DNA
Materials DNA: Höchst 33258 (High range solution): (Invitrogen H1398 or Sigma B1155 ) 1µg/mL in 1x TNE Filter and s tore at 4ºC in an amber bottle. 10x TNE stock: 100 mM Tris, 2 M NaCl, 10 mM EDTA, pH7.4. Store at room
Protein Syntheses in Cell Free Systems
the reaction mixture for protein syntheis. Incubate the reaction mixture at 37° C for 60 minutes. Terminate the reaction by pipetting 40 µl of the mixture onto a 2.5 cm disk of Whatman 3MM filter paper. Dip the disk into cold 10% TCA for 15
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