Sphingosine Kinase 1 Inhibitor

Cayman的SPHK1抑制剂筛选检测试剂盒提供了一个强大且易于使用的平台，用于识别人类SPHK1的新型抑制剂，SPHK1是一种参与控制多种生物功能的酶，包括细胞增殖、存活和分化、细胞迁移和组织侵袭以及血管生成和血管完整性。该检测使用SPHK特异性荧光底物NBD-鞘氨醇。SPHK1磷酸化该底物，导致其光谱特性发生变化，可使用荧光板读数器在激发和发射波长分别为550和584nm时轻松量化。强效且可逆的SPHK1抑制剂PF-543被列为阳性对照，其抑制SPHK1的IC50值约为60.5±34.3nM。Cayman's SPHK1 Inhibitor Screening Assay Kit provides a robust and easy-to-use platform for identifying novel inhibitors of human SPHK1, an enzyme implicated in the control of diverse biological functions including cell proliferation, survival and differentiation, cell migration and tissue invasion, and angiogenesis and vascular integrity. The assay uses an SPHK-specific fluorogenic substrate, NBD-sphingosine. SPHK1 phosphorylates this substrate, generating a shift in its spectral properties, which can be easily quantified using a fluorescence plate reader at excitation and emission wavelengths of 550 and 584 nm, respectively. The potent and reversible SPHK1 inhibitor PF-543 is included as a positive control and inhibits SPHK1 with an IC50 value of approximately 60.5 ± 34.3 nM.分子式分子量