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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
8.3 with acetic acid at room temp.Adjust volume to 500 ml.Running buffer is 1X TGOE buffer[Note that there is no Mg or EDTA in both the gel and in the running buffer.]
Preparation of Microinjection Buffer
EDTA, pH 8.0 (autoclaved) 100 mM NaCl 1 ml of 5 M NaCl (autoclaved) 1x Polyamines 50 microliters 1000x Polyamines mix Autoclaved H2O up to 50 ml NOTE: Prepare fresh and discard unused microinjection buffer.
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
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