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- 701981-12 ml
- 2025年07月21日
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文献和实验Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
-----------------------Gels:10.5 ml (20%/0.33%)acrylamide/bis acrylamide3.5 ml 10X TGOE buffer1.75 ml 50% glycerol35 microliters 0.5 M DTT20.9 ml H2O0.3 ml 10% ammonium persulfate30 microliters TEMED10X TG0E 500 ml0.25 M Tris 15.1 g Tris1.9 M glycine 71.3 g glycinepH
Preparation of Microinjection Buffer
EDTA, pH 8.0 (autoclaved) 100 mM NaCl 1 ml of 5 M NaCl (autoclaved) 1x Polyamines 50 microliters 1000x Polyamines mix Autoclaved H2O up to 50 ml NOTE: Prepare fresh and discard unused microinjection buffer.
Restriction Enzyme Buffer Most enzymes can use REact buffers; however, some are made up separately. Use fresh Milli-Q water , siliconized or sterile glassware or disposable plastic ware to make the following stock solutions up fresh (discard
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