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- 2025年07月21日
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文献和实验Quantification of Absolute Amounts of Cellular Messenger RNA by RNA-Excess Solution Hybridization
are semiquantitative, unreliable for detecting small changes in the mes- sage levels, and provide only relative values of mRNA. By contrast, DNA-excess solution hybridization (4 ) can be used to quantify abso -lute levels of mRNA. In this method a cDNA fragment
Solution and Solid-State NMR of Lipids
Lipid structure and dynamics are of first importance for cellular function. Lipids such as phosphatidyl inositol (PtdIns) are essential in signaling pathways, as they are recognition sites at the membrane surface. Their head-group or chain
Radioiodination of Cellular Proteins
) Lactoperoxidase enzyme solution (see recipe ) 30% (v/v) hydrogen peroxide stock solution
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