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- 详细信息
- 文献和实验
- 技术资料
- 库存:
1KG
- 英文名:
Borane morpholine complex
- CAS号:
4856-95-5
- 保质期:
2年
- 供应商:
湖北魏氏化学试剂
- 规格:
500G
4856-95-5
湖北魏氏化学专注科研试剂现货供应商
随货质检单、检测方法和检测图谱等技术资料
英文名称:Borane morpholine complex
CAS号:4856-95-5
分子式:C4H12BNO 分子量:100.96
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文献和实验Antibody Precipitation with Protein A or G
. Then incubate the complex-bound immobilized protein A or G for 5 minutes at 95°C with 25µl of the SDS-PAGE sample buffer. Centrifuge the sample and collect the supernatant. Repeat, pooling supernatant for a total of 50µl. The sample is now ready
Peptide Pull-Down (PPD) Assay for Identification and Characterization of Histone PTM Effectors
Incubate with rotation at room temperature for 3-5 hours to bind peptides to the beads. (See note 4) Wash bound beads at least 3 times with 1 mL PBS + 0.1% Triton X-100 to remove unbound peptide Resuspend peptide-conjugated beads in 400 µL
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
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