- ￥350
- MedChemExpress(MCE)
- 中国
- HY-42745-250mg
- 2025年07月20日
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
广州威佳科技有限公司
- CAS号：
50586-80-6
- 规格：
250mg
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文献和实验Large-scale yeast transformation大规模转化酵母【Yale University】
up and down to mix cells and PEG2 . Capmat deepwells. Incubate at 30℃ for 15-30 mins, on side. Heat shock at 45℃ for 15 mins. Pellet cells at 3000 r.p.m. for 15 mins at least. Yeast form diffuse layer on bottom of well. Tip PEG off quickly and carefully. Wipe
Large-scale yeast transformation
pellet size. Should be approx 3 ul. If much greater, use less culture for following steps 2 . Spin down cells. 2000 r.p.m., 4 minutes. Resuspend in sterile water then spin down again. Mix: 72 mls water, 9 mls 10xTE pH 7.5, 9 mls 1M LiAc
, Meurer L, Ashton-Prolla P. (2005) An alternative protocol for DNA extraction from formalin-fixed and paraffin wax-embedded tissue. J Clin Pathol. 58(8):894-5. Gilbert MTP, Haselkorn T, Bunce M, Sanchez JJ, Lucas SB, Jewell LD, Van Marck E,Worobey M
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