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文献和实验。从流行病学角度出发,在水、食物或其它外环境标本中检出NV意义重要。需注意检测技术要锁定在高度怀疑的传播媒介上,尽早采样并置4℃存放,若是饮用水,需用大容量(5~100L)浓集病毒后检测。 实验室检查 电镜法 直接电镜法(EM)和免疫电镜法(IEM),EM 法观察的灵敏度较低,要求每毫升粪便样品中至少有大约 106个病毒粒子,因此只能用于患病早期病毒大量排出时采集的样本检测。IEM 法比 EM 法的敏感性可提高 100 倍,主要应用患者恢复期血清捕捉同型抗原,从而增加检出率。电镜法
Real-Time Fluorescence Imaging of Abdominal, Pleural, and Lymphatic Metastases
-specific HSV NV1066 expresses green fluorescent protein (GFP) in infected cancer cells. One single dose of NV1066 administered via intratumor, intracavitary, or systemic injection can spread within and across body cavities to target tumor cells
Detection of Noroviruses of Genogroups I and II in Drinking Water by Real-Time One-Step RT-PCR
. A simple method for the isolation and detection of noroviruses in water is described, where both norovirus genogroups (NV gg I and NV gg II) are detected separately. The isolation of the viruses is performed by filtration of a 1-L water sample
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