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文献和实验Autoradiographic Visualization in Brain of Receptor-G Protein Coupling Using [35S]GTPS Binding
-stimulated [35 S]guanylyl-5′-O-(γ-thio)-triphosphate ([35 S]GTPγS) binding, originally developed for receptors in isolated membranes, has provided an excellent opportunity to apply this process to brain sections, thus allowing the visualization of receptor
40:59‐72. Janeba, Z., Lin, X., and Robins, M.J. 2004. Functionalization of guanosine and 2′‐deoxyguanosine at C6: A modified Appel process and SNAr displacement
[35S]GTPS Binding as an Index of Total G-Protein and G-Subtype-Specific Activation by GPCRs
preparations, GTP can be substituted by 35 S-labeled guanosine� 5′-O -(3-thio)triphosphate ([35 S]GTPγS) and on agonist stimulation a quasi-stable [35 S]GTPγS–Gα �complex forms and accumulates. Separation of [35 S]GTPγS–Gα complexes from free [35 S]GTPγS allows
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