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文献和实验A Scintillation Proximity Assay for Fatty Acid Amide Hydrolase Compatible with Inhibitor Screening
that bind to human fatty acid amide hydrolase (FAAH). This SPA assay utilizes the specific binding of [3 H]-R (+)-methanandamide (3 H-MAEA), a competitive nonhydrolyzed FAAH inhibitor, to FAAH expressing microsomes and evaluates its displacement by FAAH
Enzyme Assay Design for High-Throughput Screening
Enzymes continue to be a major drug target class for the pharmaceutical industry with high-throughput screening the approach of choice for identifying initial active chemical compounds. The development of fluorescent- or absorbance-based
Fluorescent Indicator Methods to Assay Functional CFTR Expression in Cells
of a targetable green fluorescent protein-based halide indicator (20 ). The major advantages of a fluorescence-based assay of CFTR function are sensitivity, technical simplicity, and the ability to assay function in single cells and heterogeneous cell mixtures
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