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文献和实验Chromatographic Analysis of the Escherichia coli Polysialic Acid Capsule
Polysialic acid capsules are the major virulence factors in Escherichia coli K1, K92, and groups B and C meningococci. The sialic acid monomers (2-keto-3-deoxy-5-acetamido-7,8,9-d -glycero -d -galacto -nonulosonic acids) comprising
Characterization of Protein Glycosylation
group of Ser or Thr ( O -linked, mucin type), Xylα to the hydroxyl group of Ser (proteoglycan type), GlcNAcβ to the acetamido nitrogen of Asn ( N -linked) or to the hydroxyl group of Ser ( see Chapter 2 ), and Glcβ to ceramide (glycolipids). (B) Sialic
Rapid Identification of Cloned HIV-1 Fragments
-fragment of β-galactosidase were developed (pUC vectors) (3 ,4 ). Upon induction by IPTG (isopropyl-β-D -thio-galactopyranoside), the expressed β-galactosidase could cleave X-gal (5-bromo-4-choloro-3-indoyl-β-D -galactopyranoside) and turn the colonies
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