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文献和实验Porcine brain tubulin preparation
Homogenization buffer (freshly prepared) 1L PM buffer 100 mM MgATP 1L PMG Buffer 100 mM MgGTP 1M dithiothreitol (DTT) Glutamic acid sodium salt 实验设备 Waring Blender Temperature controlled ultracentrifuge
The presence of a redundant lipolytic system of bile salt-activated lipase (BAL) and pancreatic lipase results in the concerted action of these lipolytic enzymes during intestinal dietary fat digestion processes (1 ). Because of its wide
acid (sodium salt) to the tubulin solution and stir slowly until it is dissolved. Disburse the solution into 31.5 ml ultracentrifuge tubes and pair them by weight. Immerse the centrifuge tubes to the level of the liquid within in a 37oC
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