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- 2025年07月20日
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文献和实验E.Z.N.A.® Protocol for Bacteria
实验试剂 Additional materials to be supplied by user 1. RNase-free Lysozyme 2. TE buffer (10 mM Tris-HCl, pH 7.6, 1 mM EDTA) Procedure 实验步骤 1. Harvest Cells and resuspend in 500 ul TE
E.Z.N.Z.TM MicroElute RNA Clean-up Protocol
upon addition of ethanol in Step 3. Vortex and add the entire mixture to the column. With the spin column inside a 2ml collection tube (supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection
E.Z.N.Z.TM MicroElute RNA Desalting and Concentration protocol
(supplied with kit), centrifuge at 10,000 x g for 30 seconds at room temperature. Discard flowthrough and re-use the collection tube. 5. Place column in the same 2 ml collection tube from step 4 and add 500 ul RWB Wash Buffer diluted with absolute
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