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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
CROTYLPALLADIUM CHLORIDE DIMER
- 库存:
98
- 供应商:
北京孚博
- CAS号:
12081-22-0
- 规格:
1g
CAS:12081-22-0
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文献和实验annealing temp for degenerated primer - PCR problem
PCR primers should be free of significant complementarily at their 3'-termini as this favours formation of primer-dimer which reduce product yield. Formation of primer-dimer artifacts may also cause more serious problems, such as non
rate. Larger size PCR fragment need more dNTP. (e) primers - up to 3 uM of primers may be used, but high primer to template ratio can results in non-specific amplification and primer-dimer formation (note: store primers in small aliquots). (f) Primer
recognizes both strands of the DNA (i.e. both strands must be non-methylated for recognition). It is able to do this because it is a homo-dimer protein. Restriction endonucleases Since different bacterial strains and species have potentially different R/M
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