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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Sucrose, monoallyl ether
- 库存:
98
- 供应商:
北京孚博
- CAS号:
12002-22-1
- 规格:
1g
CAS:12002-22-1
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文献和实验ARABIDOPSIS THALIANA DNA ISOLATION PROCEDURE
the tissue and stir for 3 min., pour into Buchner funnel to remove ether, rinse with cold H2O. Add 300 ml of buffer A (1 M sucrose, 10 mM Tris-HCl (pH 7.2), 5 mM MgCl2 , 5 mM 2-mercaptoethanol and 400ug/ml ethidium bromide). Grind tissue with a Polytron
Protein Syntheses in Cell Free Systems
mM -mercaptoethanol•10X solution of 20 amino acids•Teflon homogenizer•Refrigerated preparative centrifuge•Sat.(NH )SO•TBS-M plus 20% (v/v)glycerol•1X TBS-M buffer containing 0.25 M sucrose•1X TBS-M buffer containing 1.0 M sucrose•Sephadex G-25 column
An Ultra-fast method of DNA extraction from Neurospora
the need for large numbers of flasks when growing many cultures for DNA isolation. 1. Inoculate plastic Petri plates containing 25 ml of 1X Vogels, 2 sucrose, 0.04 Tween-80 (to prevent conidiation) (Zalokar, M. 1954. Arch. Biochem. Biophys. 50:71-80
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