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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
1,3-Butadiene,homopolymer
- 供应商:
北京孚博
- CAS号:
9003-17-2
- 规格:
1g
CAS:9003-17-2
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文献和实验Ligation of DNA fragments by homopolymer tailing
and ethanol precipitation).4. Prepare an annealing mixture containing vector DNA and target DNA with complementary homopolymer tails in 100 l of 1 × annealing buffer. The DNA concentration and ratio of vector to target depends on the nature of the cloning
monooxygenases (FMOs) in the metabolism and toxicity of three important industrial chemicals—1,3-butadiene (BD), trichloroethylene (TRI), and tetrachloroethylene (TETRA; also known as perchloroethylene)—as model compounds. The chapter illustrates examples
Sequential Deletions of Single-Stranded DNA: “Cyclone Sequencing”
through the insert. The 3′ end is then homopolymer tailed using terminal transferase and the original oligomer used to create the double-stranded region, which has a 3′ end complementary to the homopolymer tail, is reannealed across the restriction site
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