- 询价
- ITI Biochem
- 美国
- 117057-51-9
- 2025年07月19日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
N-Allyl-4-aminobenzenesulfonamide
- 库存：
98
- 供应商：
北京孚博
- CAS号：
117057-51-9
- 规格：
1g
CAS：117057-51-9
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文献和实验Revised amino-allyl labeling protocol
for an additional 1 hour. B. Hydrolysis 1. Degrade RNA by addition of 15 m L of 0.1 N NaOH. Incubate at 70C for 10 minutes 2. Neutralize by addition of 15 m L 0.1 N HCl. To continue with the amino-allyl dye coupling procedure
Amino-allyl Reverse Transcription
ul 1N NaOH 10ul .5M EDTA Incubate: 15 min. at 65° C. Neutralize: 25ul 1M Tris pH 7.4 or 1M HEPES pH 7.5 III. Cleanup To continue with the amino-allyl dye coupling procedure all Tris must be removed from the reaction to prevent
Protocol for Reverse Transcription and Amino-allyl Coupling of RNA
by addition of 15 mL 0.1 N HCl. To continue with the amino-allyl dye coupling procedure, all Tris must be removed from the reaction to prevent the monofunctional NHS-ester Cy-dyes from coupling to free amine groups in solution. 3. Add 450
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