- ￥4500
- 百欧泰
- BAYT-547
- 2025年08月12日
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- 保存条件：
2-8°C
- 保质期：
6个月
- 库存：
100
- 供应商：
百欧泰
- 规格：
1mg
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文献和实验Purification of recombinant sBRF M166L
culture with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml) and grow at 30 deg. for four hours. Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g
Purification of recombinant sBRF M166L
with 0.4 mM IPTG (4 ml of 0.1 M IPTG per 1000 ml)and grow at 30 deg.for four hours.Take a small sample of the induced and uninduced cultures and save for analysis of whole cell protein by SDS PAGE. Harvest cells by centrifugation (4-6g).Resuspend the cell
Purification of Antibody Light Chains by Metal Affinity and Protein L Chromatography
(five or six His residues) placed at either the C- or N-terminus of a recombinant protein can form a stable chelate with immobilized transition metals. This allows fractionation of the target protein to 90–95% purity levels in a single chromatographic step (2 –4 ). Metals
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