IA2460 阿利新蓝8GX 热点染料/探针 索莱宝

 

 

阿利新蓝（Alcian）又称爱先蓝或阿尔辛蓝等，是一种类铜钛花青共轭染料。这种阳离子染料与酸性基团结合，即阿尔辛蓝与组织内含有的阴离子基团如羧基和硫酸根形成不溶性复合物。阿利新蓝由中央含铜的酞菁环与四个异Thiourea基通过硫醚键相连而成。该异Thiourea基呈中度碱性，使阿利新蓝带阳离子。阿利新蓝使碳水化合物着色的确切机制不明，普遍认为是阳离子的异Thiourea基通过静电与组织内的多聚阴离子相连。如含羧基和硫酸根的酸性黏液物质的羧基和硫酸根形成不溶性复合物，即染料分子中带正电荷的盐键和酸性黏液物质中带负电荷的酸性基团结合呈蓝色。

利用染液的不同pH 值可区分粘液物质的类属:

pH 值为 2.5 时组织内的羧基电离， 带有一个负电荷， 与阿利新蓝中的阳离子形成盐键，是带有羧基的组织（如蛋白多糖/透明质酸以及上皮酸性黏蛋白）染色。

pH 值为 1.0 时，组织内的硫酸根电离，带有一个负电荷，与阿利新蓝中的阳离子形成盐键，使带有硫酸根的组织（如硫酸黏液物质）染色。中性黏蛋白(如胃黏膜和 Brunner 腺体部位的中性黏蛋白）不能与阿利新蓝反应。

软骨染色中：pH为1时，羧基(COOH)不着色，硫酸基(OSO3H)着色，pH为 2.5 时，羧基染色良好而硫酸粘液着色不佳，使硫酸黏蛋白和唾液黏蛋白着色。

本试剂仅适用于科研领域，不适用于临床诊断或其他用途。

 

使用方法（仅供参考）

1 改良 Lison 法∶

骨髓片甲醇固定5 min，水洗，风干;阿利新蓝液（10 g/L 阿利新蓝水溶液 100 mL和1%醋酸 100 mL 混合后过滤）染 30 min，充分水洗;1 g/L中性红水溶液复染 30 s，水洗，风干，镜检。

2 Mowry法∶

固定骨髓片5 min，水洗;阿利新蓝液（阿利新蓝1g，3%醋酸100 mL）染5～10 min，充分水洗，1 g/L中性红水渗液复染 30 s，水洗，风干，镜检。

3 Lev法∶

新鲜涂片甲醇固定5 min，水洗;阿利新蓝液（阿利新蓝1 g，0.1 mol/L盐酸 100 mL）染 10～30 min，充分水洗;1 g/L中性红水溶液复染30 s，水洗，风干，镜检。

 

In Vitro

Cell（rat bone mesenchymal stem cells (rBMSCs)）

Chondrogenic di?erentiation of rBMSCs on the prepared membranes was evaluated through alcian blue staining. After sterilization, the membrane with the size of 15 mm × 15 mm was mounted on the CellCrown transwell and ?xed into a 24-well culture plate. Cells at passage 2 were seeded on the membranes at the density of 75,000 cells per membrane and cultured for 2 days to allow for optimal attachment. Cells seeded onto the culture plate were set as the blank control. The cell culture medium was then replaced by the chondrogenic induction medium for further induction. The medium was refreshed every 2 days. After induction for 7, 14, and 21 days respectively, Alcian blue staining (Solarbio, cat# IA2460) was performed according to the manufacturer’s instructions, and the stained samples were then imaged.

来源文献：Zhu Y, Dai B, Zhang S, Liu J, Xu S, Liu W, Chen X, Zhang H, Li Q, Pang FO, Li W, Wen C, Qin L, Xu J, Ngai T. Tissue Mimetic Membranes for Healing Augmentation of Tendon-Bone Interface in Rotator Cuff Repair. Adv Mater. 2025 Jan 31:e2407358. doi: 10.1002/adma.202407358. Epub ahead of print. PMID: 39888084.

 

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标题:OTUB1 promotes osteoblastic bone formation through stabilizing FGFR2

成员:Zhu Qiong, Fu Yesheng, Cui Chun-Ping, Ding Yi, Deng Zhikang, Ning Chao, Hu Fan, Qiu Chen, Yu Biyue, Zhou Xuemei, Yang Guan, Peng Jiang, Zou Weiguo, Liu Cui Hua, Zhang Lingqiang

论文因子:38.104 发表期刊:Signal Transduction and Targeted Therapy pmid:37024477

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标题:Nrf2 activation is involved in osteogenic differentiation of periodontal ligament stem cells under cyclic mechanical stretch

成员:Xi X; Zhao Y; Liu H; Li Z; Chen S; Liu D

论文因子:3.7 发表期刊:Exp Cell Res pmid:33865812

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标题:Conditional ablation of MAPK7 expression in chondrocytes impairs endochondral bone formation in limbs and adaptation of chondrocytes to hypoxia

成员:YangX;ZhongD;GaoW;LiaoZ;ChenY;ZhangS;ZhouH;SuP;XuC

论文因子:6.068 发表期刊:Cell Biosci pmid:32944217

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标题:lncRNA-CRNDE regulates BMSC chondrogenic differentiation and promotes cartilage repair in osteoarthritis through SIRT1/SOX9

成员:Shi C. Zheng W. Wang J

论文因子:3.108 发表期刊:Mol Cell Biochem pmid:33479807

基本信息
CAS	No.33864-99-2
中文名称	阿利新蓝8GX
英文名称	Alcian Blue 8GX
分子式	C56H68Cl4CuN16S4
分子量	1298.86
溶解性	Soluble in Water ≥3mg/mL
外观(性状)	Purple to black Solid
储存条件	Store at RT
EC	EINECS 278-333-8
MDL	MFCD00010720
SMILES	CN(C)C(=[N+](C)C)SCC1=CC2=C(C=C1)C3=NC4=NC(=NC5=C6C=C(C=CC6=C([N-]5)N=C7C8=C(C=CC(=C8)CSC(=[N+](C)C)N(C)C)C(=N7)N=C2[N-]3)CSC(=[N+](C)C)N(C)C)C9=C4C=C(C=C9)CSC(=[N+](C)C)N(C)C.[Cl-].[Cl-].[Cl-].[Cl-].[Cu+2]
背景说明	是一种类铜钛花青共轭染料。
生物活性	Alcian Blue 8GX is a commonly used phthalocyanine dye that binds to glycoproteins and glycosaminoglycans. Alcian Blue 8GX has a wide range of applications in biological staining， including proteins in brain tumors and DNA in cells and tissues[1][2][3][4].
In Vitro	Isolation and culture of cranial NCCs in vitro facilitates screening and analyses of molecular cellular mechanisms of cranial NCCs implicated in craniofacial development. Here， we present a method for the isolation and culture of cranial NCCs harvested from the first branchial arch at early embryonic stages. Morphology of isolated cranial NCCs was similar to O9-1 cells， a cell line for neural crest stem cells.Moreover， cranial NCCs isolated from a transgenic mouse line with enhanced bone morphogenetic protein (BMP) signaling in NCCs showed an increase in their chondrogenic differentiation capacity， suggesting maintenance of their in vivo differentiation potentials observed in vitro.[1]
数据来源文献	[1]. Hiroki Ueharu， et al. Isolation and Culture of Cranial Neural Crest Cells from the First Branchial Arch of Mice. Bio Protoc. 2022 Apr 5;12(7):e4371. [2]. J Tas， et al. The Alcian blue and combined Alcian blue--Safranin O staining of glycosaminoglycans studied in a model system and in mast cells. Histochem J. 1977 Mar;9(2):205-30. [3]. Brian G Redfern， et al. An alternative Alcian Blue dye variant for the evaluation of fetal cartilage. Birth Defects Res B Dev Reprod Toxicol. 2007 Jun;80(3):171-6. [4]. Yuan Fang Li， et al. Determination of Proteins Based on Their Resonance Light Scattering Enhancement Effect on Alcian Blue 8GX. Original Papers. 2000 Dec; VOL.16
单位	瓶