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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Sophoricoside(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
152-95-4
- 规格：
1ml
| 基本信息 | |
| CAS | No.152-95-4 |
| 中文名称 | 槐角苷(10mM in DMSO,无菌） |
| 英文名称 | Sophoricoside(10mM in DMSO,Sterile） |
| 分子式 | C21H20O10 |
| 分子量 | 432.38 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | Others |
| 通路 | Others |
| 背景说明 | Sophoricoside 是一种槐花中的异黄酮糖苷，具有抗炎，抗癌和免疫抑制活性。 |
| 生物活性 | Sophoricoside is an isoflavone glycoside isolated from Sophora japonica and has anti-inflammatory， anti-cancer and immunosuppressive effects.[1] |
| In Vitro | Sophoricoside is an isoflavone glycoside isolated from Sophora japonica and has anti-inflammatory， anti-cancer and immunosuppressive effects. The results show that Sophoricoside (50 μM) significantly inhibits the PMACI-induced histamine release. The inhibition rate reaches up to 30.24%. The maximal rates of TNF-α， IL-8 and IL-6 inhibition by Sophoricoside (50 μM) are approximately 31.42%， 43.43% and 34.24%， respectively. The rates of the levels of Rel/p65 inhibition in nuclear by Sophoricoside (50 μM) is approximately 50.14%. Results show that the enhanced caspase-1 activity induced by PMACI is significantly reduced by Sophoricoside in a dose-dependent manner[1]. |
| 细胞实验 | When the Sophoricoside is orally administered 1 h before compound 48/80 injections， the scratching behaviors is reduced. The inhibition rate of Sophoricoside (2 mg/kg) is approximately 41.21%. Orally administered Sophoricoside inhibits the scratching behaviors by 47.31%. When mice are treated for 2 weeks with Sophoricoside， the atopic dermatitis is recovered to a significant extent[1]. |
| 细胞实验 | To test the cell viability by each concentration of Sophoricoside， the MTT colorimetric assay is performed. Briefly， HMC-1 cells (3×105 cells/well) are incubated with Sophoricoside (1 to 50 μM) for 12 h. After the addition of MTT solution， the cells are incubated at 37°C for 4 h. The crystallized MTT (formazan) is dissolved in dimethyl sulfoxide and the absorbance is measured at 540 nm[1]. |
| 动物实验 | BALB/c mice (5 weeks， 19 to 20 g) are used in this study. The dorsal skin of the BALB/c mice (n=6) is shaved and treated with a depilatory prior to the experiment. The mice are sensitized with 100 μL of 0.15% DNCB in acetone-olive oil (3:1) applied to the dorsal skin twice per week for 5 weeks. Control mice receive vehicle (acetone/olive oil=3:1). After 3 weeks， Sophoricoside (2 mg/kg) is orally administered 2 weeks until the end of the experiment[1]. |
| 激酶实验 | Cells are incubated with the Sophoricoside for 1 h， and then incubated with PMACI for 2 h. The reaction is stopped by cooling the tubes in ice. The cells are separated from the released histamine by centrifugation at 400×g for 5 min at 4°C. The histamine levels are measured by ELISA using a histamine assay kit according to the manufacturer’s directions. Duplicate aliquots of supernatant are measured for each sample[1]. |
| 数据来源文献 | [1]. Kim SJ， et al. The ameliorative effect of sophoricoside on mast cell-mediated allergic inflammation in vivo and in vitro. Molecules. 2013 May 22;18(5):6113-27. |
| 单位 | 瓶 |
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IS07301 槐角苷(10mM in DMSO,无菌） 抑制剂/拮抗剂/激动剂 索莱宝
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