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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Diosimin(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
520-27-4
- 规格：
1ml
| 基本信息 | |
| CAS | No.520-27-4 |
| 中文名称 | 地奥司明(10mM in DMSO,无菌） |
| 英文名称 | Diosimin(10mM in DMSO,Sterile） |
| 分子式 | C28H32O15 |
| 分子量 | 608.54 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | AhR(Aryl Hydrocarbon Receptor) |
| 通路 | Immunology & Inflammation |
| 背景说明 | Diosmin 是芳烃受体 (AhR) 的激动剂。 |
| 生物活性 | Diosmin is a flavonoid found in a variety of citrus fruits and also an agonist of the aryl hydrocarbon receptor (AhR).[1-2] |
| In Vitro | Treatment with Diosmin causes a dose dependent increase in the amount of adducts formed (up to a 7-fold increase in adducts at 5 μM Diosmin). At 5 μM， Diosmin increases the cytotoxicity of 7，12-dimethylbenz(a)anthracene， shifting the IC50 from an estimated 1.2 μM to 400 nM. Diosmin is not cytotoxic in itself at the concentrations tested. Diosmin causes an increase in CYPIAI activity in MCF-7 cells in a time- and dose-dependent fashion. Diosmin causes a dose-dependent increase in CYPIAI mRNA after 24 h of incubation， causes a long-lasting increase in CYPIAI mRNA accumu lation that reaches its peak after 48 h of incubation[1]. |
| 细胞实验 | Diosmin significantly decreases the malondialdehyde (MDA) levels and increases the activities of total-superoxide dismutase (T-SOD)， glutathione peroxidase (GSH-Px)， and catalase (CAT) in the retina of rats compare with the ischemia group (P[2]. |
| 细胞实验 | MCF-7 cells are plated at 25，000 cells/well in 24-well plates. After 24 h， the medium is changed to medium containing 5 μM Diosmin. After an additional 24 h， the medium is again changed with medium containing 5 μM Diosmin. After 3 days， the total cell growth is assessed by sulforhodamine[1]. |
| 动物实验 | Healthy male Wistar rats (n=112) weighing 180 to 200?g each are used in this study. The animals are randomly assigned to the following 4 groups， which include combinations of the ischemia/reperfusion (I/R) injury model or sham injury with the i.g. administration of Diosmin or vehicle solution: sham+vehicle (SV) group， sham+Diosmin (SD) group， model+vehicle (MV) group， and model+Diosmin (MD) group. For intragastric administration， 5?mL of 2% Diosmin per kilogram weight of the rat， or the same volume of vehicle solution， is administered intragastrically 30?min before the onset of ischemia， and then daily after I/R injury until the animals are sacrificed. Using an overdose of anesthesia， 8 rats from each group are sacrificed 24?h after I/R injury， and their eyeballs harvested for determination of the malondialdehyde (MDA) level and the activities of total-superoxide dismutase (T-SOD)， glutathione peroxidase (GSH-Px)， and catalase (CAT). At 7 days post-I/R injury， electroretinograms (ERGs) are recorded in 6 rats per group. Meanwhile， 6 rats in each group are randomly chosen for retrograde labeling of retinal ganglion cells (RGCs) ， and the remaining 8 rats from each group are histopathologically examined[2]. |
| 数据来源文献 | [1]. Ciolino HP， et al. Diosmin and diosmetin are agonists of the aryl hydrocarbon receptor that differentially affectcytochrome P450 1A1 activity. Cancer Res. 1998 Jul 1;58(13):2754-60. [2]. Tong N， et al. Diosmin protects rat retina from ischemia/reperfusion injury. J Ocul Pharmacol Ther. 2012 Oct;28(5):459-66. |
| 单位 | 瓶 |
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