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- IA06801
- 2025年07月23日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
5-Azacytidine(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
320-67-2
- 规格：
0.1ml/0.3ml/0.5ml/1.5ml/1ml
| 规格： | 0.1ml | 产品价格： | ￥200.0 |
|---|---|---|---|
| 规格： | 0.3ml | 产品价格： | ￥300.0 |
| 规格： | 0.5ml | 产品价格： | ￥400.0 |
| 规格： | 1.5ml | 产品价格： | ￥650.0 |
| 规格： | 1ml | 产品价格： | ￥500.0 |
| 基本信息 | |
| CAS | No.320-67-2 |
| 中文名称 | 阿扎胞苷(10mM in DMSO,无菌） |
| 英文名称 | 5-Azacytidine(10mM in DMSO,Sterile） |
| 分子式 | C8H12N4O5 |
| 分子量 | 244.2 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | DNA Methyltransferase;Autophagy |
| 通路 | Epigenetics;Autophagy |
| 背景说明 | 5-Azacytidine是一种胞苷核苷类似物，也是一种DNA 甲基化抑制剂，通过与 DNA 结合共价捕获 DNA甲基转移酶 (DNA methyltransferases)特异性抑制DNA甲基化。5-Azacytidine 通过降低 DNA 甲基化水平调节基因表达。 |
| 生物活性 | 5-Azacytidine (Azacitidine; 5-AzaC; Ladakamycin) is a nucleoside analogue of cytidine that specifically inhibits DNA methylation. 5-Azacytidine is incorporated into DNA to covalently trap DNA methyltransferases and contributes to reverse epigenetic changes. 5-Azacytidine induces cell autophagy.[1-4] |
| In Vitro | 5-Azacytidine was first synthesized almost 40 years ago. It was demonstrated to have a wide range of anti-metabolic activities when tested against cultured cancer cells and to be an effective chemotherapeutic agent for acute myelogenous leukemia.The finding that 5-azacytidine was incorporated into DNA and that， when present in DNA， it inhibited DNA methylation， led to widespread use of 5-azacytidine and 5-aza-2-deoxycytidine (Decitabine) to demonstrate the correlation between loss of methylation in specific gene regions and activation of the associated genes. [1] |
| 细胞实验 | Twenty mL of cells (circa 1×104 cells/mL) are pipetted into sterilized culture tubes with screw caps and incubated at 37°C overnight. The experiment is initiated by the addition of 1 mL of 5-Azacytidine (5-azaCR) or medium for a given period (from 0 to 240 min) prior to the addition of 1 mL of metabolite (or medium). Cell growth is determined twice a day for 3 days by means of a Model A Coulter counter. To determine IDSO and ID90 values， 5 mL of L1210 cells (5×103 cells/mL) are incubated with the drug at 37°C for 3 days， and cell growth is determined.[3] |
| 动物实验 | For the in vivo experiments， leukemic mice (bearing circa 1×103 cells/animal) are given injections i.p. with 0.2 mL of 5-Azacytidine (5-azaCR) of a given concentration. Two hr later， the reaction is started by injecting 0.5 mL of labeled metabolite (TdR-3H or UR-3H， 10 /μCi/12.5 μg). After 1 hr， animals (3 mice/group) are killed by cervical fracture.[3] |
| 激酶实验 | A crude cell-free extract is isolated from LI 210 cells in culture by suspension of the cells in a given volume of 0.05mol/LTris-HCl buffer， pH 7.4， and sonic extraction with a Biosonik at 70% maximal output for 30 sec. The supernatant is collected after centrifugation at 105，000 × g for 60 min (4°C) in a Model L Spinco ultracentrifuge. The final protein concentration of the cell-free extracts is approximately 3 mg/mL. The extracts are used as the source of enzymes. Ribonucleotide reductase activity is measured. A unit of enzyme is defined as the amount that catalyzed dCMP synthesis at a rate of 1 mμmole/hr. The assay systems for the measurement of pyrimidine nucleoside (CR) and deoxynucleoside (TdR， CdR) kinases are essentially those described by Chu and Fischer. However， reactions are terminated by heating for 2 min in a boiling water bath， and the phosphorylated derivatives are isolated according to the method of Bach. Fifty-jul aliquots are applied to 1-inch discs of diethylaminoethyl paper， which are then placed in counting vials and eluted with 0.5 mL of 0.5 mol/LPCA. After 1 hr， 12 mL of Diotol are added， and the radioactivity is determined.[3] |
| 数据来源文献 | [1]. Christman JK. 5-Azacytidine and 5-aza-2-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy. Oncogene. 2002 Aug 12;21(35):5483-95. [2]. Creusot F， et al. Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidineand 5-aza-2-deoxycytidine. J Biol Chem. 1982 Feb 25;257(4):2041-8. [3]. Li LH，et al. Cytotoxicity and mode of action of 5-azacytidine on L1210 leukemia. Cancer Res. 1970 Nov;30(11):2760-9. [4]. Marycz K， et al. 5-Azacytidine?and?Resveratrol?Enhance?Chondrogenic?Differentiation?of?Metabolic?Syndrome-Derived?Mesenchymal?Stem?Cells?by?Modulating?Autophagy.Oxid Med Cell Longev.?2019 May 12;2019:1523140. |
| 单位 | 瓶 |
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文献和实验相关实验
体，通常为10mm的茎段和直径10mm的叶片部分（太大激素作用减弱，太小则不易成活）。 消毒注意事项：1、表面消毒剂对植物组织是有害的，应正确选择消毒剂的浓度和处理时间，以减少组织的死亡。2、在表面消毒后，必须用无菌水漂洗材料3次以上以除去残留杀菌剂，但若用酒精消毒，则不必漂洗。3、与消毒剂接触过的切面在转移到无菌基质前需将其切除，因为消毒剂会阻碍植物细胞对基质中营养物质的吸收。4、若外植体污染严重则应先用流水漂洗1小时以上或先种子培养得到无菌种苗，然后用其各个部分建立组织培养。5、HgCl2效果
玻片上作薄膜涂片；尸检或感染动物组织，病变局部涂抹采样的棉拭子直接涂片。固体培养基上的菌落或菌苔的制片，先用接种环取一环生理盐水置载玻片中央，再用无菌接种环取少量的培养物在生理盐水中磨匀，涂布成1cm2大小的涂面，置室温下自然干燥或远火慢慢烘干。 2．固定 目的是杀死细菌，凝固细菌蛋白及结构，便于染色；促使细菌粘附在载玻片上，避免在水洗过程中被水冲掉；改变细菌对染料的通透性，有利于菌细胞内结构的染色。通常用火焰加热固定，将已干燥的涂片在火焰中迅速通过3次，以手背皮肤接触玻片不烫为佳
子(Transformant,即带有异源DNA分子的受体细胞)。目前常用的感受态细胞制备方法有CaCl2和RbCl(KCl)法，RbCl(KCl)法制备的感受态细胞转化效率较高,但CaCl2 法简便易行,且其转化效率完全可以满足一般实验的要求,制备出的感受态细胞暂时不用时,可加入占总体积15％的无菌甘油于-70℃保存(半年),因此CaCl2法为使用更广泛。为了提高转化效率, 实验中要考虑以下几个重要因素： 1. 细胞生长状态和密度: 不要用经过多次转接或储于４℃的培养菌，最好从－70℃或-20℃甘油保存的菌种中
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IA06801 阿扎胞苷(10mM in DMSO,无菌） 表观遗传学 索莱宝
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