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- IS00401
- 2025年07月23日
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- 详细信息
- 技术资料
- 保存条件：
Stroe at -20℃，6 months.
- 保质期：
Stroe at -20℃，6 months.
- 英文名：
Sanguinarine Chloride(10mM in DMSO,Sterile）
- 库存：
现询
- 供应商：
北京索莱宝科技有限公司
- CAS号：
5578-73-4
- 规格：
1ml
| 基本信息 | |
| CAS | No.5578-73-4 |
| 中文名称 | 氯化血根碱(10mM in DMSO,无菌） |
| 英文名称 | Sanguinarine Chloride(10mM in DMSO,Sterile） |
| 分子式 | C20H14NO4Cl |
| 分子量 | 367.78 |
| 溶解性 | 请根据自己的实验要求使用。 |
| 外观(性状) | 无菌溶液 |
| 储存条件 | Stroe at -20℃，6 months. |
| 靶点 | Apoptosis |
| 通路 | Apoptosis |
| 背景说明 | Sanguinarine Chloride是一种生物碱，可通过激活活性氧 (ROS) 的产生来刺激细胞凋亡。诱导的细胞凋亡与 JNK 和 NF-κB 的活化有关。 |
| 生物活性 | Sanguinarine (Sanguinarin) chloride， a benzophenanthridine alkaloid derived from the root of Sanguinaria Canadensis， can stimulate apoptosis via activating the production of reactive oxygen species (ROS). Sanguinarine-induced apoptosis is associated with the activation of JNK and NF-κB.[1] |
| In Vitro | Sanguinarine (SANG)-induced apoptosis is associated with the activation of JNK and NF-κB signal pathways.To determine the effects of Sanguinarine on cell viability， 22B-cFluc cells are stimulated with different concentrations of Sanguinarine for 24 h， and then a CKK-8 assay is performed. The treatment with Sanguinarine decreases the proliferation of 22B cells in a dose-dependent manner. Meanwhile， the cytosolic extracts of 22B-cFluc cells treated with different dose of Sanguinarine are measured to detect cellular caspase-3 activity using Ac-DEVD-pNA， which is a validated caspase-3 substrate. The absorbance at 450 nm increases in a dose-dependent manner， indicating increased caspase-3 activity stimulated by Sanguinarine[1]. |
| 细胞实验 | To evaluate the apoptosis induced by Sanguinarine (SANG) in vivo， 22B-cFluc cells are inoculated subcutaneously into one flank of nude mice and xenograft models are allowed to establish. Mice are treated intravenously with 10 mg/kg of Sanguinarine. At 24， 48 and 72 h after treatment， bioluminescent imaging is performed after i.p. injection of mice with 150 mg/kg of D-luciferin substrate. Sanguinarine treatment induces an obvious increase of luminescent signal as early as 48 h after initial treatment. A sustained bioluminescent imaging (BLI) intensity increased is observed throughout the course of experiment. At 72 h after treatment， the tumors are collected and subjected to TUNEL staining for evaluating apoptosis. Compared with the control tumors， the group treated with Sanguinarine exhibits significantly more cell apoptosis， indicated by the increased green signals from the sporadic apoptotic cells[1]. |
| 细胞实验 | The cell viability of Sanguinarine is determined by CCK-8 assay using a cell counting kit-8. Briefly， 22B-cFluc cells are seeded in a 96-well plate (5×103 cells/well) and treated with different concentrations of Sanguinarine (0.5 μM， 1 μM， 2 μM， 4 μM) for 24 h. Then 10 mL CKK-8 is added to each well for 4 h and the absorbance at 450 nm is measured with a microplate reader. The optical density (OD) values are determined to reflect the viable cell populations from each well[1]. |
| 动物实验 | Mice[1] Xenografted tumor models are prepared by injection of 1×107 22B-cFluc cells suspended in PBS into nude mouse (n=6). After tumors reach a volume of approximately 100 mm3， Sanguinarine (10 mg/kg) is i.v. injected into mice. After injection for 24 h， 48 h and 72 h， mice are given a single i.p. dose of 150 mg/kg D-luciferin and bioluminescence imaging are performed using a Xenogen Lumina II system. The signal intensity in the region of interest is expressed using the Living Image software 4.1. For the anti-tumor therapy studies， one group of tumor-bearing mice (n=6) receive intravenously 10 mg/kg of Sanguinarine every other day throughout the experimental period， while the control group of mice (n=6) receive DMSO only. Tumor growth measurement is calculated[1]. |
| 激酶实验 | The caspase-3 activity is measured using a caspase-3 activity assay kit. Briefly， the cells treated by different concentrations of Sanguinarine (0.5 μM， 1 μM， 2 μM， 4 μM) or control DMSO are collected， washed and lysed in a lysis buffer for 30 min on ice. The supernatants are then collected by centrifuging at 1，2000 rpm for 10 min. The Ac-DEVD-pNA (2 mM) is added to each sample and incubated at 37°C for 1 h. The optical density (OD) of each sample is finally quantified at a wavelength of 405 nm using a spectrophotometer. The p-NA standard is used to calibrate the caspase-3 activity of each sample[1]. |
| 数据来源文献 | [1]. Wang Y， Noninvasive bioluminescence imaging of the dynamics of sanguinarine induced apoptosis via activation of reactive oxygen species. Oncotarget. 2016 Apr 19;7(16):22355-67. |
| 单位 | 瓶 |
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IS00401 氯化血根碱(10mM in DMSO,无菌） 抑制剂/拮抗剂/激动剂 索莱宝
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