Benzo[b]thiophene-2-carboxamid

[3H](+)MK801 Radioligand Binding Assay at the N‐Methyl‐D‐Aspartate Receptor

., Siegel, B.W., Harrison, B.L., Gross, R.S., Hawes, C., and Towers, P. 1996. [3H]MDL 105,519, a high‐affinity radioligand for the N‐methyl‐D‐aspartate receptor‐associated glycine recognition site J. Pharmacol. Exp. Ther. 279:62‐68

Library cDNA Synthesis

  Library cDNA Synthesis 1° cDNA Synthesis N.B: During 1° cDNA synthesis, all steps should be carried while wearing gloves and all solutions should either have been DEPC-treated or purchased specifically for use with RNA.

Characterization of Histaminergic Receptors

) to measure nonspecific binding (Table 1.19.2 ) 1 nM [3 H]N ‐α‐methyl histamine (NAMH; 45 to 90 Ci/mmol; Perkin‐Elmer Life Sciences; Table 1.19.3 ) in Tris/EDTA assay buffer (see recipe