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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Benzenesulfonic acid, 2,5-dihydroxy-3-methoxy- (4CI)
- 库存:
98
- 供应商:
北京孚博
- CAS号:
675140-68-8
- 规格:
1g
CAS:675140-68-8
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文献和实验The dipeptidic dihydroxyethylene isostere (27, 3R , 4R , 5S )-5-tert -butyloxy-carbonylamino-3,4-dihydroxy-2-isopropyl-3,4-O,O -isoprop-ylidene-6-cyclohexyl-hexanoic acid, 15 , is a representative building block of an important class
with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml. 4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA
] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA concentration is in the nM range. 5) Incubate cells with label for a period of 60 minutes (2 hours???). 6) Remove label by washing cells 3 X with ice cold media
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