ACETAMIDE,N-[2-[CYCLOPROPYL(IS

Determination of Sulfur Amino Acids, Glutathione, and Related Aminothiols in Biological Samples by Gas Chromatography with Flame Photometric Detection

. Although GC method based on the preparation of N-heptafluorobutyryl isobutyl or ethyl esters (8 ) is selective and sensitive by flame photometric detection (FPD), this method is not applied to the analysis of biological samples. As the HPLC methods

Expression and Purification of Recombinant -Defensins and -Defensin Precursors in Escherichia coli

by centrifugation are lysed in 6 M guanidine-HCl under non-reducing conditions, the expressed defensin fused to its 6xHis-34 amino acid N-terminal fusion partner is purified by affinity chromatography on nickel-NTA columns. A Met codon introduced at the N terminus

Synthesis of Triazole‐Nucleoside Phosphoramidites and Their Use in Solid‐Phase Oligonucleotide Synthesis

N ‐(2‐(tert ‐Butyldimethylsilyloxy)ethyl)‐uracil‐1‐yl‐N ‐(prop‐2‐ynyl)acetamide (15 ; protocol 3 ) Ethyl 2‐(N ‐(2‐azidoethyl)‐uracil‐1‐yl‐acetamido)acetate (16