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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
5-BROMO-2-(2,2,2-TRIFLUORO-ETHOXY)-PYRIMIDINE
- 库存:
98
- 供应商:
北京孚博
- CAS号:
433683-47-7
- 规格:
1g
CAS:433683-47-7
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文献和实验A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation
The thymidine analogue 5-bromo-2′-deoxyuridine (BrdU) has been widely used to make sister chromatid differentiation (SCD) evident in metaphase chromosomes of cells grown for two cycles in BrdU and, thus, containing varying
, or other biologically active molecules. In this chapter, we present a technique, which allows identification of specific DNA sequences by fluorescence in situ hybridization (FISH) and simultaneously analyses the incorporation of a thymidine analogue, 5-bromo
. A spectrofluorometric method is described for the determination of CYP2B6-catalyzed 7-ethoxy-4-trifluoromethylcoumarin O -deethylation activity in human liver microsomes. The specificity of this method for CYP2B6 is increased by the use of inhibitory antibodies to CYP
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