5-AMINO-2-CHLORO-N,N-DIMETHYL-

N-Terminal Amino Acid Sequencing of 2-DE Spots

Two-dimensional electrophoresis (2-DE) is one of the most advanced techniques for direct separation of considerable numbers of proteins. Direct microsequence analysis of the amino (N)-terminal partial sequence of the electroblotted protein

Amino Acid Analysis Using Precolumn Derivatization with 6-Aminoquinolyl-N-Hydroxysuccinimidyl Carbamate

Over the past 20 years, amino acid analysis methods based on precolumn derivatization procedures have become a popular alternative to the traditional postcolumn derivatization techniques based on ion-exchange separation of the underivatized

Incorporation of Unnatural Non--Amino Acids into the N-Terminus of Proteins in a Cell-Free Translation System

, the incorporation of unnatural α-amino acids and non-α-amino acids into the N-terminus of proteins has been achieved using expanded initiation codons. Here, we describe the method for the incorporation of fluorescent-labeled non-α-amino acids into the N-terminus