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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
4-Ethyl-4'-ethynylbiphenyl
- 库存:
98
- 供应商:
北京孚博
- CAS号:
477587-89-6
- 规格:
1g
CAS:477587-89-6
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文献和实验Cell cycle analysis of Escherichia coli cells
: The C+D period is estimated from the initiation age (ai ), the generation time (τ) and the number of generations spanned per cell cycle. Example: 4-origin-cells: 23 % Generation time (τ): 27 min Initiation age (ai ): 5 min
Phycoerythrin conjugation protocol
(Molecular Probes) DTT Dithiothreitol NEM N-ethyl maleimide * 10 ml Sephadex G-10 columns (2) Separation column A long Sephacryl S-300 column
for 2-3'. 7. Resuspend in 100 µl 0.25M Tris pH 7.8 8. Freeze-thaw 3x (10'freeze-10'thaw). 9. Spin down debris @14K RPM for 5'. 10. Transfer 100 µl supernatant to a new set of tubes. 11. Set new set of tubes and add 40 µl of supernatant to each. 12. In separate
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