4-Chloro-2-(1H-imidazol-1-yl)-

Phenol chloro form Extraction of DNA

    Materials: phenol:chloroform (1:1) chloroform Add an equal

Small scale His-Tag fusion protein purification under denaturative conditions

of the sonicator) 4   Spin 15min max speed 4°C 5   Transfer supernatant into clean tube: crude extract (keep 40µl for PAGE-SDS) see Equilibration of Ni-NTA agarose ) 7   Add the crude extract to the beads and incubate 4°C / 1h (swirl

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

for the etherification at the O 6 ?position of silyl?protected inosine, guanosine, and 2??deoxyguanosine is described. Typically, a THF solution of the silylated nucleoside is treated with 1H ?benzotriazol?1?yloxy?tris(dimethylamino)phosphonium hexafluorophosphate (BOP