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文献和实验Biochem 88:138-143, 2003; Genome Res 16:1075-1083, 2006). In order to capture this type of information from primary human tissues, we have adopted and optimized the HELP assay (HpaII tiny fragment Enrichment by Ligation-mediated PCR) to compare
Methylation analyis using restriction enzyme digestion
. Since in eukaryotic DNA (or actually in mammalian DNA) only cytosine in CG context can be methylated, the restriction enzymes with CG sequences within their restriction sites come in question. Two classical enzyme pairs are HpaII - MspI (CCGG) and SmaI - XmaI (CCCGGG
Methylation analyis using restriction
very carefully.If you'll just take genomic DNA,digest it with HpaII,blot and hybridise with selected probe the result will be very complicated.With 200 bp probe you can obtain myriad of bands which you will not be able to interpret.Therefore first of all define
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