人血清白蛋白

In Vitro

荧光猝灭实验（500 μg/mL HSA solutions，37 °C for 1 h）
HSA solutions (500 μg/mL)were prepared in PBS buffers at various pH values (0.01 M, pH 7.4, 7.0, 6.5, 6.0, 5.5, 5.0, or 4.5). For fluorescence quenching studies, after incubating at 37 °C for 1 h, the fluorescence spectra were recorded using a fluorescence spectrometer . Heat-denatured HSA (100 °C,10 min) served as a positive control, and a PBS buffer (0.01 M, pH 7.4) was used as a negative control. For interaction studies, HSA solutions (1 mg/mL)with different pH levels were mixed with equal volumes of SMN solutions at 10, 20, 50, 100, 200, or 500 μg/mL.After incubating at 37 °C for 1 h, the changes in HSA fluorescence spectra were measured. The excitation wavelength was set to 280 nm with a slit width of 3 nm. SMN solutions at the same concentrations were used as controls.
文献来源：Ge Y, Fu F, Gao Y, He T, Mailänder V, Crespy D, Landfester K, Jiang S. Physiological pH Transition-Driven Protein Corona Dynamics Regulate Cellular Uptake and Inflammatory Responses of Silica Nanoparticles. Adv Sci (Weinh). 2025 Sep 3:e02788. doi: 10.1002/advs.202502788. Epub ahead of print. PMID: 40902197.

紫外-可见光谱实验（500 μg/mL HSA solutions，37 °C for 1 h）

HSA solutions (500 μg/mL)were prepared in PBS buffers at various pH values (0.01 M, pH 7.4, 7.0, 6.5, 6.0, 5.5, 5.0, or 4.5). After incubating at 37 °C for 1 h, the UV–vis spectra were recorded

using a UV-vis spectrophotometer . Heat-denatured HSA (100 °C, 10 min) served as a positive control and PBS buffer (0.01 M, pH 7.4) was used as negative control. For interaction studies, HSA solutions (1 mg/ mL)with different pH levels were mixed with equal volumes of SMN solutions (200 μg/mL). After incubating at 37 °C for 1 h, the UV–vis spectra of HSA were measured using a UV–vis spectrophotometer , with spectra ranging from 200 to 500 nm. SMN solution (100 μg/mL)was used as the reference solution.
文献来源：Ge Y, Fu F, Gao Y, He T, Mailänder V, Crespy D, Landfester K, Jiang S. Physiological pH Transition-Driven Protein Corona Dynamics Regulate Cellular Uptake and Inflammatory Responses of Silica Nanoparticles. Adv Sci (Weinh). 2025 Sep 3:e02788. doi: 10.1002/advs.202502788. Epub ahead of print. PMID: 40902197.

圆二色谱（CD）实验（250 μg/mL HSA solutions,37 °C for 1 h）
HSA solutions (250 μg/mL)were prepared in PBS buffers at various pH values (0.01 M, pH 7.4, 7.0, 6.5, 6.0, 5.5, 5.0, or 4.5). After incubating at 37 °C for 1 h, CD spectra were measured using

aCD spectropolarimeter (Jasco, J-815) in a quartz cuvette (path length =0.1 cm, scanning range = 190–260 nm). Measurements were conducted three times (digital integration time = 2s, bandwidth = 1nm, data interval = 0.5 nm, scanning speed = 100 nm min−1). The PBS buffer (0.01 M, pH 7.4) was used for baseline correction. Heat-denatured HSA (250 μg/mL,100 °Cfor 10 min) served as a positive control. The CD spectra were analyzed for secondary structure content using the CDSSTR algorithm in CDPro software. For interaction studies, HSA solutions (500 μg/mL)with different pH levels were mixed with equal volumes of SMN solutions (100 μg/mL). After incubating at 37 °C for 1 h, the CD spectra of HSA were measured under the same conditions as described above. SMN solution (50 μg/mL)served as a negative control. All experiments were conducted under N2 (0.4 MPa).

文献来源：Ge Y, Fu F, Gao Y, He T, Mailänder V, Crespy D, Landfester K, Jiang S. Physiological pH Transition-Driven Protein Corona Dynamics Regulate Cellular Uptake and Inflammatory Responses of Silica Nanoparticles. Adv Sci (Weinh). 2025 Sep 3:e02788. doi: 10.1002/advs.202502788. Epub ahead of print. PMID: 40902197.