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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
2-Aminomethyl-3-(2-bromophenyl)propionic acid HCl
- 库存:
98
- 供应商:
北京孚博
- CAS号:
910443-85-5
- 规格:
1g
CAS:910443-85-5
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文献和实验Protein Gel Preparation and Staining
Tris Base pH to 8.8 with HCl 0.4% SDS 0.4 g SDS up to 100 ml with Q 4X Stacking Gel Buffer 0.5 M Tris pH 6.8 6.06 g Tris Base pH to 6.8 with HCl 0.4% SDS 0.4 g SDS up to 100 ml with Q 1X Glycine Running Buffer
【资源】How dose an SDS-PAGE really work?
the following: SDS (sodium dodecyl sulphate, also called lauryl sulphate), b-mercaptoethanol (BME), bromophenol blue, glycerol, and Tris-glycine at pH 6.8. BME is added to prevent oxidation of cysteines and to break up disulfide bonds. Bromophenyl blue is a dye
Gel Mobility Shift Assay Conditions -Mg/EDTA in Gel and Buffer
50% glycerol100 mM Tris-HCl pH8 @ 25 degrees 100 microliters 1 M Tris pH8300 mM KCl 300 microliters 1 M KCl25 mM MgCl2 25 microliters 1 M MgCl2500 micrograms/ml BSA 5 microliters 100 mg/ml BSA170 microliters H2Ooptional: add 25 microliters saturated
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