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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Spexyte™ Intracellular pH Calibration Buffer Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
100Tests
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文献和实验Simultaneous Analysis of Intracellular pH and Ca2+ from Cell Populations
by pH, as indicated above. We describe procedures to correct for this effect and to obtain calibration parameters for fura-2 and snarf-1 required to facilitate analysis of pH and Ca2+ concentrations within cell populations.
实验试剂 1. Sterile deionized water (or TE buffer) 2. Absolute (or 95%) ethanol 实验设备 1. Sterile 1.5 mL centrifuge tubes. 2. Protective eye-ware 实验步骤 1. MicroElute™ DNA
Buffer P1 50 mM Tris-HCl pH 8.0 10 mM EDTA 100 μg/ml RNaseA The buffer and RNaseA can also be ordered from Qiagen separately (catalog numbers 19051 and 19101). Buffer P2 200 mM NaOH 1% SDS Buffer P3
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