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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
1,4-diethynybenzene and1,3-diethynybenzene
- 供应商:
北京孚博
- CAS号:
30523-88-7
- 规格:
1g
CAS:30523-88-7
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文献和实验Brdu免疫组织化学染色分析 BrdU incorporation assay
Then in 5% normal goat serum in PBS with 1% BSA at RT for 1 hour. Mouse anti-BrdU diluted to 1:1000 with 1% NGS and1%BSA in PBS, incubateat 4C overnight. Rinse with PBS 3x. Goat Biotinylated anti-Mouse IgG 1:200 in 1% NGS, 1%BSA in PBS
,NJ), loaded with Cu 2+ . 3. Binding Buffer: 0.5 M NaCl, 20 mM Tris-HCl, pH 7.5, and1% (w/v) CHAPS. 4. Elution Buffer: Binding Buffer with 100 mM imidazole, pH 7.5. 5. Syringe (10 mL) with 0.2-μm pore size filter. 2.3.3. Step 3: Gel
) solution in waterand freeze aliquots at –20°C. 5. Water-saturated isobutanol. Store at RT. 6. Running buffer (10×): 250 mM Tris, 1.92 mM glycine, and1% (w/v) SDS. Store at RT. 7. Prestained molecular weight markers: Rainbow marker (GEHealthcare
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