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- 文献和实验
- 技术资料
- 英文名:
(+/-)-1-(4-BROMOPHENYL)ETHYL ISOCYANATE
- 供应商:
北京孚博
- CAS号:
207974-15-0
- 规格:
1g
CAS:207974-15-0
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文献和实验A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
Phycoerythrin conjugation protocol
(Molecular Probes) DTT Dithiothreitol NEM N-ethyl maleimide * 10 ml Sephadex G-10 columns (2) Separation column A long Sephacryl S-300 column
until bromophenyl blue is about 3/4 of the way to the bottom; ~1.5 hrs for a 10% gel). 2. Visualize the oligonucleotides using UV shadowing. Place the gel on saran wrap and place on top of an intensifying screen. Shining short wave UV light from a hand held lamp
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