Lipase Activity Assay Kit

脂肪酶通过控制富含甘油三酯的脂蛋白从循环系统中清除，在膳食脂质的消化、运输和处理中发挥着重要作用。操纵脂肪分解对经常与肥胖有关的代谢紊乱具有治疗潜力。Cayman的脂肪酶活性测定法提供了一种基于荧光的方法来检测血浆、血清、组织匀浆和细胞培养样品中的脂肪酶活性。在测定中，脂肪酶将花生四烯酰-1-硫代甘油水解为花生四烯酸和硫代甘油。硫代甘油与硫醇荧光检测器反应产生强荧光产物，可以用380-390nm的激发波长和510-520nm的发射波长进行分析。Lipases perform essential roles in the digestion, transportation, and processing of dietary lipids by controlling the clearance of triglyceride-rich lipoproteins from the circulation. Manipulating lipolysis has therapeutic potential in the metabolic disorders frequently associated with obesity. Cayman's Lipase Activity Assay provides a fluorescence-based method for detecting lipase activity in plasma, serum, tissue homogenates, and cell culture samples. In the assay, lipase hydrolyzes arachidonoyl-1-thioglycerol to arachidonic acid and thioglycerol. Thioglycerol reacts with the thiol fluorometric detector to yield a highly fluorescent product which can be analyzed with an excitation wavelength of 380-390 nm and an emission wavelength of 510-520 nm.分子式分子量