COX Fluorescent Activity Assay

Cayman的COX荧光活性测定法提供了一种方便的荧光法，用于检测粗酶制剂（细胞裂解物/组织匀浆）和纯化酶制剂中的COX-1或COX-2活性。该测定法利用COX的过氧化物酶成分。在该测定法中，PGG2和ADHP之间的反应产生高荧光化合物试卤灵，可以使用530-540nm之间的激发波长和585-595nm之间的发射波长进行分析。该试剂盒包括同工酶特异性抑制剂，用于区分COX-2活性和COX-1活性。Cayman's COX Fluorescent Activity Assay provides a convenient fluorescence-based method for detecting COX-1 or COX-2 activity in both crude (cell lysates/tissue homogenates) and purified enzyme preparations. The assay utilizes the peroxidase component of COXs. In this assay, the reaction between PGG2 and ADHP produces the highly fluorescent compound resorufin that can be analyzed using an excitation wavelength between 530-540 nm and an emission wavelength between 585-595 nm. The kit includes isozyme-specific inhibitors for distinguishing COX-2 activity from COX-1 activity.分子式分子量