Cyclic AMP ELISA Kit (without

cAMP是一种普遍存在的细胞第二信使，是连接膜受体及其配体与激活细胞内酶活性和基因表达的信号转导途径的重要组成部分。cAMP由膜结合腺苷酸环化酶从ATP合成，并通过促进其磷酸化或去磷酸化来激活或抑制各种酶或酶级联。Cayman的cAMP检测是一种竞争性ELISA，可在0.078-10pmol/ml的标准曲线范围内测量cAMP，乙酰化cAMP的灵敏度(80% B/B₀)通常为0.1pmol/ml。非乙酰化cAMP的范围为0.3-750pmol/ml，灵敏度(80% B/B₀)约为3pmol/ml。cAMP is a ubiquitous cellular second messenger that is a critical component of a signal transduction pathway linking membrane receptors and their ligands to the activation of internal cellular enzymatic activity and gene expression. cAMP is synthesized from ATP by membrane-bound adenylate cyclase and activates or inhibits various enzymes or cascade of enzymes by promoting their phosphorylation or dephosphorylation. Cayman's cAMP assay is a competitive ELISA that permits cAMP measurements within the standard curve range of 0.078-10 pmol/ml, typically with a sensitivity (80% B/B₀) of 0.1 pmol/ml for acetylated cAMP. Non-acetylated cAMP has a range from 0.3-750 pmol/ml and a sensitivity (80% B/B₀) of approximately 3 pmol/ml.分子式分子量