Human Therapeutic IgG1 ELISA K

Cayman的人类治疗性IgG1 ELISA试剂盒是一种免疫测定（即夹心）检测方法，可用于定量猴、大鼠、小鼠或兔血浆和血清中的人类IgG1，无需事先纯化样品。标准曲线范围为3.13-200ng/ml，定量下限(LLOQ)为6.25ng/ml。该检测方法已在食蟹猴、恒河猴、大鼠、小鼠和兔血清中得到验证。虽然以人类单克隆IgG1抗体作为标准品，但我们承认，我们的标准品和客户正在评估的人类IgG1测试品之间可能存在蛋白质结构或翻译后修饰的细微差异，这些差异可能导致测试品定量不准确。因此，我们预计许多客户将更愿意使用他们自己纯化的治疗性单克隆IgG1作为此ELISA中的标准品。在这种情况下，我们建议客户在ELISA中以与试剂盒中提供的标准相似的剂量范围测试他们自己的标准。Cayman's Human Therapeutic IgG1 ELISA Kit is an immunometric (i.e. sandwich) assay that can be used for the quantification of human IgG1 in monkey, rat, mouse, or rabbit plasma and serum without prior sample purification. The standard curve spans the range of 3.13-200 ng/ml, with a lower limit of quantification (LLOQ) of 6.25 ng/ml. The assay has been validated in serum from cynomolgus monkey, rhesus monkey, rat, mouse, and rabbit.Although a human monoclonal IgG1 antibody is supplied as a standard, we acknowledge that minor differences in protein structure or post-translational modification may exist between our standard and the human IgG1 test article that is being assessed by the customer, and that these differences could lead to inaccuracies in the quantification of the test article. Thus, we anticipate that many of our customers will prefer to use their own purified therapeutic monoclonal IgG1 as the standard in this ELISA. In that event, we recommend that the customer test their own standard in the ELISA at a similar dose range as the standard provided in the kit.分子式分子量