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999
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100 ug
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文献和实验secondary antibody review -- data from 99 publications
localization of tagged SAX-3 in 293T cell Molecular Probes 2 Alexa Fluor 647 immunocytochemistry localization of BCG phagosomes 3 Alexa Fluor 647 immunohistochemistry detect surface DFz2 3 FITC immunohistochemistry determine
antibody. The amount will depend on the antibody and the abundance (total and relative) of the antigen, but a good starting point is 0.5μg antibody / 500μg lysate. 8) Incubate for 1-2hr at 4OC with rocking. Avoid overnight incubationsa. 9) Add 30-40μl
Single-molecule analysis of DNA replication by molecular combing
is then detected with a monoclonal antibody directed against BrdU and DNA fibers are counterstained with an antibody against single-stranded DNA. Replicating DNA fibers are revealed with fluorescent secondary antibodies and are imaged with an epifluorescence
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