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文献和实验Characterization of Nuclear Receptor Ligands by Multiplexed Peptide Interactions
., and McDonnell, D.P. 1999. Peptide antagonists of the human estrogen receptor. Science 285:744‐746. Northrop, J.P., Nguyen, D., Piplani, S., Olivan, S.E., Kwan, S.T., Go, N.F., Hart, C
[3H](+)MK801 Radioligand Binding Assay at the N‐Methyl‐D‐Aspartate Receptor
protocol that detects ligand activity at the NMDA receptor?associated glycine site. This is a convenient approach that exploits the ability of NMDA receptor modulators to alter the kinetics of ligands that bind to the channel?blocking site of the NMDA
Analysis of Retinoid Receptor Phosphorylation
as O -phosphorylation (3 ). Indeed, the most common biochemical techniques, from gel fixing to peptide analysis and purification, rely on the use of low pH solutions in which N-phosphorylated residues are unstable. These technical aspecis have so far
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